Title of article :
Liquid–vapor interfacial tension of blood plasma, serum and purified protein constituents thereof
Author/Authors :
Anandi Krishnan، نويسنده , , Arwen Wilson، نويسنده , , Jacqueline Sturgeon، نويسنده , , Christopher A. Siedlecki، نويسنده , , Erwin A. Vogler، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Pages :
9
From page :
3445
To page :
3453
Abstract :
A systematic study of water–air (liquid–vapor, LV) interfacial tension γlv of blood plasma and serum derived from four different mammalian species (human, bovine, ovine and equine) reveals nearly identical concentration-dependence ( ; where CB is plasma/serum dilution expressed in v/v concentration units). Comparison of results to a previously-published survey of purified human-blood proteins further reveals that of plasma and serum is surprisingly similar to that of purified protein constituents. It is thus concluded that any combination of blood-protein constituents will be substantially similar because of individual proteins are very similar. Experimental results are further interpreted in terms of a recently-developed theory emphasizing the controlling role of water in protein adsorption. Accordingly, the LV interphase saturates with protein adsorbed from bulk solution at a fixed weight-volume concentration ( 436 mg/mL) independent of protein identity or mixture. As a direct consequence, of purified proteins closely resembles that of mixed solutions and does not depend on the relative proportions of individual proteins comprising a mixture. Thus variations in the plasma proteome between species are not reflected in nor is serum different from plasma in this regard, despite being depleted of coagulation proteins (e.g. fibrinogen). A comparison of pendant-drop and Wilhelmy-balance tensiometry as tools for assessing protein γlv shows that measurement conditions employed in the typical Wilhelmy plate approach fails to achieve the steady-state adsorption state that is accessible to pendant-drop tensiometry.
Keywords :
protein adsorption , Liquid–vapour interfacial tension , Plasma , serum , BLOOD PROTEINS
Journal title :
Biomaterials
Serial Year :
2005
Journal title :
Biomaterials
Record number :
546169
Link To Document :
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