Title of article :
Radioiodination of mouse anti-III β-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies
Author/Authors :
Helena ?vecov?، نويسنده , , Veronika Kleinov?، نويسنده , , Daniel Seifert، نويسنده , , Hana Chaloupkov?، نويسنده , , J?rg B?urle، نويسنده , , Karel Kranda، نويسنده , , Vlastimil Kr?l، نويسنده , , Miroslav Fi?er، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Abstract :
The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125I in order to develop new imaging agents against the specific neuronal marker III β-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
Keywords :
III ?-tubulin , Peripheral neuropathies , Radioiodination , scFv , 125-iodine , MAb
Journal title :
Applied Radiation and Isotopes
Journal title :
Applied Radiation and Isotopes
