Nutrient Medium Optimization for Rosmarinic Acid Production by Lavandula vera MM Cell Suspension

The overall effect of NH4NO3, KNO3, and KH2PO4 on the biosynthesis of rosmarinic acid and cell biomass by Lavandula vera MM cell suspension was studied by the method of the full factor experiment. Polynomial regression models were elaborated to give a quantitative description of the processes of biosynthesis of rosmarinic acid (Y1) and cell biomass (Y2) as a result of the variation of the concentration of NH4+, 0.09 g/L <- X1 <- 0.23 g/L; NO3-, 2.44 g/L <- X2 <- 3.02 g/L; and K2PC4 0.170 <- X3 <0.425 g/L. Optimization procedures according to the modified Simplex method allowed us to establish the optimal conditions for the biosynthesis of rosmarinic acid by Lavandula vera MM: X1 = 0.09 g/L; X2 - 3.02 g/L, and X3 == 0.170 g/L, where Y1 max - 1786.74 mg/L (27 times higher compared with the cultivation in the standard Linsmayer-Skoog medium). As a result, modified ingredients of the Linsmayer-Skoog nutrient medium were applied for the cultivation of Lavandula vera MM to achieve a maximum yield of rosmarinic acid.