Title of article :
Persistence of sulphonamide resistance in Escherichia coli in the UK despite national prescribing restriction
Author/Authors :
Virve I Enne، نويسنده , , David M Livermore and on behalf of the GRASP collaboration، نويسنده , , Peter Stephens، نويسنده , , Lucinda MC Hall، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Abstract :
Background
There is a clear association between heavy antimicrobial consumption within a population and the frequent recovery of resistant bacteria, but whether a reduction in antimicrobial use can reverse this process is less clear. We investigated the effect of a national restriction of sulphonamide prescribing in the UK on the prevalence of sulphonamide resistance in Escherichia coli.
Methods
Consecutive clinical isolates of E coli were collected at the Royal London Hospital in 1991 and 1999. These collections, each of more than 350 isolates, were compared. Minimum inhibitory concentrations of sulphamethoxazole and eight other antimicrobials were determined. The presence and locations of sulphonamide-resistance genes were examined by PCR, plasmid extraction, Southern hybridisation, and transconjugation.
Findings
Despite a huge decrease in sulphonamide prescriptions (from 320 000 prescriptions per year in 1991 to 7 000 in 1999), the frequency of resistance remained high in 1999 (165/359 [46·0%] vs 143/360 [39·7%] in 1991; difference 6·2% [95% CI -0·9 to 13·3]). Integronborne sulI was present in 16·4% of isolates in 1991 and 17·5% in 1999. The prevalence of sulII increased from 26·7% in 1991 to 36·5% in 1999 (difference 9·8% [3·1 to 16·5] p=0·0046). SulII was located on large plasmids, at least some of which were conjugative multiresistance determinants.
Interpretation
These results show that a huge decrease in antibiotic prescribing does not necessarily reduce resistance within a useful time. The main reason seems to be the genetic linkage of the index resistance to other resistance determinants.
Journal title :
The Lancet
Journal title :
The Lancet