Author/Authors :
Atsushi Sameshima، نويسنده , , Toshinobu Fujiyoshi، نويسنده , , Sukjai Pholampaisathit، نويسنده , , Masato Ushikai، نويسنده , , Motoko Kono، نويسنده , , Soontorn Antarasena، نويسنده , , Katsunori Fukuda، نويسنده , , Shigeru Furata، نويسنده , , Shunro Sonoda، نويسنده , , Masaru Ohyama، نويسنده ,
Abstract :
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand, with the human papillomavirus (HPV) type-11 being the most widespread. In this study, we isolated the HPV type-11 (HPV-11) genome from subjects with RRP and subcloned the E6 and L2 open reading frames (ORFs) with the expression vectors pEX1 and pEX3. The recombinant E6/β-gal and L2/β-gal fusion proteins were expressed in E. coli. Using the recombinant proteins, we demonstrated the presence of antibodies against HPV-11 E6 and L2 in RRP patients by Western blot analysis. The prevalence of seropositivity for HPV-11 E6 and L2 were 5% (1/20) and 10% (2/20), respectively. Although RRP is caused by infection on the mucosal surface, it appears that an immune response occurs against viral proteins expressed in the epithelial lesions.
