Title of article :
Platelet-activating factor (PAF) induced cytokine production and otitis media with effusion (OME) in the rat middle ear
Author/Authors :
Manabu Ogura، نويسنده , , Masayuki Furukawa، نويسنده , , Naoki Tada، نويسنده , , Hiroki Ikeda، نويسنده , , Toshio Yamashita، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
6
From page :
338
To page :
343
Abstract :
Objectives We examined the effect of platelet-activating factor (PAF) on IL-8 production in cultured rat middle ear epithelial cells (RMECs), and the concentrations of cytokine, PAF, and PAF-acetylhydrolase (PAF-AH) were also examined in the PAF-induced experimental OME (otitis media with effusion) of rats. Methods Using an enzyme-linked immunospecific assay, we measured the levels of cytokines in the cultured RMECs and the PAF-induced OME of rats. The PAF was quantitated by the platelet-aggregating activity and the PAF-AH was measured by radioimmunoassay. Results Both PAF and C-PAF, which is a stable analogue of PAF, significantly induced production of IL-8 in the RMECs in a dose-dependent manner. The PAF-induced IL-8 production was abolished by co-incubation with WEB2170, a specific PAF receptor antagonist. The concentrations of the cytokines and PAF in the PAF-induced OME of rats were higher on day 1 and the PAF and cytokine levels seemed to correspond well with the persistence of OME. Conclusion PAF may stimulate the local production of cytokines and may induce OME in the middle ear.
Keywords :
Platelet-activating factor , cytokine , PAF-acetylhydrolase , Otitis media with effusion
Journal title :
Auris Nasus Larynx
Serial Year :
2008
Journal title :
Auris Nasus Larynx
Record number :
568052
Link To Document :
بازگشت