Title of article
Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
Author/Authors
Donguk Kim، نويسنده , , Tae-young Roh، نويسنده , , Jieun Lee، نويسنده , , Joo-yoon Noh، نويسنده , , Young-Joo Jang، نويسنده , , Kwang-Lae Hoe، نويسنده , , Hyang-Sook Yoo، نويسنده , , Myung-Un Choi، نويسنده ,
Pages
6
From page
409
To page
414
Abstract
We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
Keywords
phospholipase d , cDNA sequence , expression , (Brassica oleracea var. capitata)
Journal title
Astroparticle Physics
Record number
568194
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