Title of article :
The plasma membrane polarity of human biliary epithelial cells: in situ immunohistochemical analysis and functional implications
Author/Authors :
Jean-Yves Scoazec، نويسنده , , Annie-France Bringuier، نويسنده , , Juan F. Medina، نويسنده , , Eduardo Mart?nez-Ans?، نويسنده , , Danielle Veissiere، نويسنده , , Gérard Feldmann، نويسنده , , Chantal Housset، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1997
Pages :
11
From page :
543
To page :
553
Abstract :
Background/Aims: In transporting epithelia, like the biliary epithelium, most plasma membrane proteins present a polarized distribution, essential for the maintenance of the structural and functional properties of the epithelium. We therefore analyzed the expression of polarized plasma membrane proteins by human biliary epithelial cells in order to compare them with other transporting epithelial cells and to search for differences in plasma membrane protein expression between their different anatomical subsets. Methods: We designed an in situ immunohistochemical study of the various anatomical compartments of the human biliary tract in order to assess the pattern of expression of selected polarized plasma membrane proteins, including integrin receptors, ectopeptidases, membrane transporters and GPI-linked proteins. Results: All biliary epithelial cells expressed the same repertoire of integrins, except for integrin chain α5, restricted to the intra-hepatic compartments. All biliary epithelial cells expressed the following apical ectopeptidases: aminopeptidase-N, neutral-endopeptidase, dipeptidyl-peptidase IV. All biliary epithelial cells epxressed the mebrane transporter Na+K+-ATPase, restricted to the basolateral domain, and the apical transporters CFTR and MDR-1. The apical AE2 anion exchanger was restricted to the small intra-hepatic bile ducts and the gallbladder. The GPI-linked protein protectin was basolateral in the intrahepatic bile ducts and apical in the gallbladder. Conclusions: The structural organization of the plasma membrane of biliary epithelial cells is very similar to that of other simple epithelia and exhibits a limited degree of heterogeneity.
Keywords :
Plasma membrane proteins , polarity , Simpleepithelial cells. , Human biliary epithelial cells , immunohistochemistry , membrane transport , plasma membrane
Journal title :
Journal of Hepatology
Serial Year :
1997
Journal title :
Journal of Hepatology
Record number :
583670
Link To Document :
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