Title of article :
Activation of NF-kappaB, AP-1 and STAT transcription factors is a frequent and early event in human hepatocellular carcinomas
Author/Authors :
Ping Liu، نويسنده , , Elisabeth Kimmoun، نويسنده , , Agnès Legrand، نويسنده , , Alain Sauvanet، نويسنده , , Claude Degott، نويسنده , , Bernard Lardeux، نويسنده , , Dominique Bernuau، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Abstract :
Background/Aims: Hepatitis B and C viruses, two inducers of hepatocarcinomas, have been shown to activate AP-1, NF-kappaB and STAT in vitro, but no detailed information on the activity of these transcription factors in vivo have been provided.
Methods: We have measured the DNA binding activity of these transcription factors in the peri-tumoral and the tumoral parts of 15 primary liver cancers, of viral or non-viral etiologies, and in five hepatic metastases using electrophoretic mobility shift assays.
Results: AP-1, NF-kappaB and STAT binding activities were increased in the peritumoral tissue, compared with histologically normal livers in 73, 87 and 70%, respectively, of the cases. A further activation of AP-1, NF-kappaB, but not STAT binding in the tumoral parts was detected in 40 and 80%, respectively, of the cases. A close correlation was found between JunD and c-Jun levels and AP-1 binding activity at the tumoral stage. By contrast, AP-1 and NF-kappaB binding activities were low or only slightly elevated in the peri-tumoral and the tumoral tissue of metastases.
Conclusions: Early activation of AP-1, NF-kappaB and STAT contributes probably to the acquisition of a transformed phenotype during hepatocarcinogenesis, whatever the etiology.
Keywords :
Nuclear Factor Kappa B , AP-1 , Fos , Jun
Journal title :
Journal of Hepatology
Journal title :
Journal of Hepatology