Limiting the proliferation and reactivity of retinal Müller cell during experimental retinal detachment: the value of oxygen upplementation Original Reearch Article

PURPOE: To ae the role of hypoxia in inducing the proliferation, hypertrophy, and dyfunction of Müller cell in detached retina and the effectivene of upplemental oxygen in limiting thee reaction. METHOD: Retinal detachment were produced in the right eye of each of 13 cat; the cat urvived urgery for 3 day, during which ix were kept in normoxia (room air, 21%) and even in hyperoxia (70% oxygen). Retina were labeled for proliferation with an antibody (MIB-1) to a cell cycle protein (Ki-67), for evidence of hypertrophy employing antibodie to the intermediate filament protein glial fibrillary acidic protein (GFAP) and to β-tubulin and for diturbance of glutamate neurochemitry employing antibodie to glutamate to a glutamate receptor (GluR-2) and to glutamine ynthetae. REULT: Reult from the two animal kept in normoxia after retinal detachment confirmed previou report that detachment caued the proliferation of Müller cell, the hypertrophy of Müller cell procee, and the diruption of glutamate recycling by Müller cell. Oxygen upplementation during detachment reduced Müller cell proliferation and hypertrophy and reduced the abnormalitie in the ditribution of glutamate, GluR-2, and glutamine ynthetae. CONCLUION: Oxygen upplementation reduced the reaction of retinal Müller cell to retinal detachment, limiting their proliferation and helping to maintain their normal tructure and function. In the clinical etting, oxygen upplementation between diagnoi and reattachment urgery may reduce the incidence and everity of glial-baed complication, uch a proliferative vitreoretinopathy.