Pigment epithelium-derived factor i deficient in the vitreou of patient with choroidal neovacularization due to age-related macular degeneration

PURPOE: Pigment epithelium-derived growth factor (PEDF) i a potent inhibitor of angiogenei that i found in the normal eye. The purpoe of thi tudy i to report decreaed level of PEDF in the vitreou of eye with choroidal neovacularization (CNV) due to age-related macular degeneration (AMD). DEIGN: Propective cae-control tudy. METHOD: In a propective cae-control tudy, undiluted vitreou wa collected from nine eye of nine patient with CNV due to AMD and from an age-matched control group of 12 eye of 12 patient with retinal diorder not involving neovacularization. Vitreou PEDF and vacular endothelial growth factor (VEGF) concentration were determined by Wetern blot analye and enzyme-linked immunoorbent aay (ELIA), repectively. Angiogenic activitie of the vitreou ample were aeed in vitro uing an endothelial cell chemotaxi aay. REULT: In vitreou ample from nine eye with CNV due to AMD the mean ± D PEDF level wa 2.8 ng/μl ± 1.3 ng/μl. In vitreou ample from 12 age-matched control eye the mean ± D PEDF level wa 16.4 ng/μl ± 7.1 ng/μl. The difference between the two group wa tatitically ignificant (P = .00003). No ignificant difference in vitreou VEGF concentration wa een between CNV/AMD ample and control ample (P = .23). All CNV/AMD vitreou ample induced endothelial cell migration in vitro. No ample from age-matched non-age-related macular degeneration control could induce endothelial cell migration, and 11 of 12 were able to block VEGF-induced migration in vitro. Thi inhibitory activity required active PEDF. CONCLUION: The vitreou of patient with CNV due to AMD contained lower level of PEDF and lacked the antiangiogenic activity of vitreou from age-matched control. Thi ugget that lo of PEDF create a permiive environment for CNV patient with AMD.