Expreion of ephrinB2 and it receptor on fibroproliferative membrane in ocular angiogenic dieae

Purpoe To determine whether ephrinB2 play a role in ocular angiogenei, we invetigated the expreion of ephrinB2 and EphB receptor on retinal fibroproliferative membrane. Deign Experimental tudy of the expreion of ephrinB2 and EphB receptor within fibroproliferative membrane in patient with ocular angiogenic dieae collected during vitrectomy. Method Fibroproliferative membrane were obtained at the time of vitrectomy from 20 patient with proliferative diabetic retinopathy (PDR) and from 40 patient who had tage 5 retinopathy of prematurity. pecimen were invetigated with immunohitochemitry uing polyclonal antibodie directed againt ephrinB2 and the EphB2, EphB3, and EphB4 receptor. Immunoreactivity for von Willebrand factor (factor VIII) and α-mooth mucle actin (α-MA) wa alo determined to confirm the identity of the target vacular endothelial cell. Reult Poitive taining for ephrinB2 wa oberved on fibroproliferative membrane that were obtained from patient with PDR (65.0%) and retinopathy of prematurity (25.0%). pecifically, ephrinB2 wa found to be preent on endothelial cell, a confirmed by it colocalization with factor VIII and α-MA taining. EphB2 and EphB3 expreion wa oberved on fibroproliferative membrane that were harveted from patient with PDR (EphB2, 90.0%; EphB3, 70.0%) and retinopathy of prematurity (EphB2, 35.0%; EphB3, 45.0%). However, EphB4 expreion wa not oberved in any of the membrane derived from patient with PDR or retinopathy of prematurity. The rate of ephrinB2 expreion in patient with PDR wa ignificantly higher than that een in patient with retinopathy of prematurity, which probably reflected difference in the vacular denity of their fibroproliferative membrane. Concluion Thee data ugget that the ephrinB2-EphB2/B3 ytem may play an important role in ocular angiogenei.