Induction of apoptoi in rat retinal cell culture by partially fluorinated alkane

Purpoe To tet whether the partially fluorinated alkane (PFA) perfluorobutylbutane (O44), perfluorohexylethan (O62), and the oligomer OL62HV, recently propoed a artificial vitreou replacement (AVR), have pro-apoptotic effect in rat retinal culture. Deign Laboratory invetigation. Method Rat retinal cell culture were eeded onto microporou inert to tudy AVR-cell interaction without impairing cell urvival. Cell were treated for 24 hour with O62, O44, and OL62HV. Apoptoi wa analyzed by tranferae-mediated dUTP-biotin nick end-labeling aay and Hoecht tain. Reult O44 and O62 did not affect tructural organization and cell urvival in retinal cell culture; however, OL62HV induced increaed apoptoi compared with control culture. Concluion OL62HV, a high-vicoity PFA, induce evere retinal damage in human eye, although it uccefully paed animal experimentation. Our in vitro tudy howed a remarkable pro-apoptotic effect of OL62HV and ugget that in vitro tet can contribute to AVR biocompatibility aement.