Prevalence of taphylococcu epidermidi train With Biofilm-Forming Ability in Iolate From Conjunctiva and Facial kin

Purpoe To compare the prevalence of biofilm-forming train of taphylococcu epidermidi in the conjunctival and facial kin microflora. Deign Experimental tudy. Method The prevalence of biofilm-forming ability of 10 epidermidi train obtained from the conjunctival ac of healthy volunteer wa compared with 40 train obtained from the facial kin of healthy volunteer. The ability to form biofilm wa determined by the preence of the icaA gene, and the production of biofilm wa examined by qualitative (Congo red agar [CRA]) and quantitative (microtiter plate) aay. Additionally, the prevalence of 36 epidermidi train obtained from the conjunctival ac of precataract patient to form biofilm wa invetigated. Reult The icaA gene wa detected in 60% of the iolate from the conjunctival ac of volunteer and 15% of thoe from the facial kin. Fifty percent of the iolate from the conjunctiva of volunteer and 5% from the facial kin were CRA poitive. Biofilm production wa ignificantly greater in iolate from the conjunctiva of volunteer. Of the nine pair of iolate found in the ame volunteer, ix conjunctival ac iolate were poitive for the icaA gene with biofilm-forming ability except one train, wherea only one of the facial kin iolate wa poitive for the icaA gene and none exhibited biofilm-forming phenotype. ixty-nine percent and 44% of the iolate from the conjunctival ac of precataract patient were poitive for icaA gene and CRA tet, repectively. Concluion The prevalence of biofilm-forming epidermidi iolate i higher in the conjunctival ac than the facial kin.