Microdiection Genotyping Analyi of the Effect of Intraarterial Cytoreductive Chemotherapy in the Treatment of Lacrimal Gland Adenoid Cytic Carcinoma

Purpoe To invetigate the feaibility of integrating molecular analyi into tandard hitopathology for lacrimal gland adenoid cytic carcinoma (ACC), and to gain inight into the molecular pathogenei of thi tumor and it repone to intraarterial cytoreductive chemotherapy (IACC) that i of clinical ue. Deign A retropective, comparative cae erie. Method etting: Intitutional. patient population: Nine conecutive patient with lacrimal gland ACC were treated with IACC, followed by orbital exenteration and chemoradiotherapy. Thi cae erie wa compared with a erie of even patient treated by conventional local therapie. intervention procedure: Gene analyi wa performed on microdiected tiue ample. Mutational allelotyping targeting nine genomic loci wa performed with 15 polymorphic microatellite marker ituated in proximity to known tumor uppreor gene erving a marker for the preence of gene deletion. main outcome meaure: A fractional mutation index wa ued to compare the acquired mutational load between different tumor having nonidentical pattern of microatellite informativene. Reult Allelic imbalance (lo of heterozygoity [LOH]) for microatellite marker at 1p36 wa the ingle mot common ite affected by imbalance in thi erie, followed by LOH in temporal equence involving 9p21, 22q12, 10q23, and 9q22. Concluion Microdiection genotyping hold promie a a clinical tool in integrating molecular analyi into tandard hitopathology to advance the undertanding of lacrimal gland ACC tumorigenei. A unique time coure for temporal mutation acquiition in ACC i propoed, coniting of 1p36 lo firt. Allelic lo for microatellite marker at 1p36 may be a common a well a an early event in ACC formation and progreion.