Burst production of superoxide anion in human endothelial cells by lysophosphatidylcholine Original Research Article

This study examined whether lysophosphatidylcholine (lysoPC), an atherogenic lipid, may stimulate production of O√2− in cultured human endothelial cells. Production of O√2− was detected by bis-N-methylacridinium nitrate (lucigenin)-elicited chemiluminescence. LysoPC was found to induce burst production of O√2−, peaked at 2not, vert, similar4 min after the stimulation, in intact endothelial cells. LysoPC also stimulated NADH-dependent production of O√2− in particulate fraction of the cells, and the action of lysoPC was inhibited by diphenyliodonium. The results suggested that lysoPC stimulated production of O√2− partly through membrane-associated NADH-dependent O√2− production systems.