Quantification of immunoglobulins and cytokines in human cervical mucus during each trimester of pregnancy

Objectives: Our aims were to determine immunoglobulin and cytokine levels in cervical mucus obtained from women during each trimester of pregnancy and to compare these levels with those reported in normally menstruating women and in women taking oral contraceptives. Study Design: Cervical mucus samples were collected at specified intervals from 36 pregnant women. An enzyme-linked immunosorbent assay was used to quantitate the presence of immunoglobulins A and G and interleukins 1β, 6, and 10 in cervical mucus. Results: Immunoglobulin A in cervical mucus remained stable during each trimester of pregnancy (26 mg/dL). Cervical mucus immunoglobulin G decreased from a first-trimester high of 44.4 mg/dL to lower levels in the second and third trimesters. Levels of interleukin 1β increased significantly from the first trimester (4261 pg/mL) to 12,899 pg/mL in the second trimester (P< .01). Conclusion: These data suggest a possible correlation of reproductive hormones and immunologic factors in the female reproductive tract during pregnancy. (Am J Obstet Gynecol 2001;184:865-74.)