Alkaloids contents of Hyoscyamus niger L. at different organs in different growth stages

Hyoscyamus niger L. is an important medicinal plant belonging to Solanaceae family. This plant is a rich source of medicinal substances including tropane alkaloids. In this investigation, we studied effects of different concentrations of nitrogen (0, 12.5, 27.5, 41, 55, 69, 83 mg in each pot) on growth and Tropane production. After the extraction of Tropanes, they were analyzed by TLC and HPLC. The results from TLC showed that roots had the highest content of hyoscyamine and scopolamine. In roots and leaves, scopolamine was the major alkaloid. Control plants had higher contents of hyoscyamine and scopolamine than treated plants. The plants treated with nitrogen had higher fresh weight and dry weight. On the other hand, increased nitrogen concentration increased root and leaf biomass. With HPLC analysis, scopolamine and hyoscyamine retention times obtained 9 and 11 min, respectively.