From Experimental Approaches to Computational Techniques: A Review on the Prediction of Protein-Protein In teractions

A crucial step towards understanding the properties of cellular systems in organisms is to map their network of protein-proteininteractions (PPIs) on a proteomic-wide scale completely and as accurately as possible. Uncovering the diverse function of proteinsand their interactions within the cell may improve our understanding of disease and provide a basis for the development of noveltherapeutic approaches. The development of large-scale high-th roughput experiments has resulted in the production of a largevolume of data which has aided in the uncovering of PPIs. However, these data are often erroneous and limited in interactomecoverage. Therefore, additional experimental and computationa l methods are required to accelerate the discovery of PPIs. Thispaper provides a review on the prediction of PPIs addressing key prediction principles and highlighting the common experimentaland computational techniques currently employed to infer PPI networks along with relevant studies in the area.