Introducing aldolase C as a differentiation biomarker: A proteomics approach

Aldolase C as a glycolytic enzyme is associated with cellular structure at developmental stages of all cells, and this is particularly evident during the early stages of morphogenesis. It seems that expression of aldolase C can be regulated by the rate of differentiation that depends on the level of transcription or mRNA stability. There are several techniques to detect gene expression, here proteomics was used for determining expression of aldolase C (as a differentiation factor) in several cell types and basal cell carcinoma (BCC) tissue. The human astrocytes were differentiated from mesanchymal stem cells, fibroblast cells were cultured as primary cell culture and BCC tissue was taken from the patient. The fibroblast cells divided into two groups including sham and exposed groups. The exposed cells are them that were exposed to continue Extremely Low-Frequency Electromagnetic Fields (ELF-EMF). The analysis of 2DE gels, showed different expression of aldolase C in mentioned cells. The findings indicate that the amount of aldolase C expression decreases as differentiation process develops.