Changes of ovarian fluid compositions and sperm quality parameters in koi (ornamental Cyprinus carpio) during spawning season

The purpose of the present study was to analyze changes of ovarian fluid, seminal plasma compositions, also sperm production characteristics (spermatocrit, sperm density and sperm volume) and sperm motility traits (sperm movement duration and percentage of motile spermatozoa) during spawning season (February to May) in koi, Cyprinus carpio (Linnaeus, 1758). Ovarian fluid and semen samples were collected from 10 female and 15 male fish in each month respectively. The results suggested that percentage of motile spermatozoa, sperm movement duration and sperm volume had not significantly changes (P>0.05), but spermatocrit and sperm density shown significant changes (P>0.05) among February, March, April and may. On the other hand, no significant differences were recorded in ovarian fluid compositions. Analysis seminal plasma compositions shown that Sodium, glucose and total protein significantly changed (P<0.05) between four term, and other seminal plasma compositions had not changes (P>0.05) during spawning season. The present study described that evaluation of these parameters will help in development of the basic knowledge about reproduction biology in koi.