Herbicidal Activity of Mycelial and Cell Free Extracts of Fusarium oxysporum F.sp. Ciceris Against Rice Weed Cyperus Iria l

The present study was undertaken to evaluate herbicidal activity of Fusarium oxysporum f.sp. Ciceris isolated from infected cowpea mycelial and cell free extracts against rice weed Cyprus iria L adopting leaf necrosis assay. Extracts were prepared from fungal mycelia and mycelia free filtrate derived from modified Fries media, sabouraud maltose yeast extract broth, sabouraud sucrose yeast extract broth,sabouraud dextrose broth and potato dextrose broth and extracted with different solvents. Chloroform and ethanol mycelial extract from modified fries media and Chloroform, methanol mycelia free filtrate extract from sabouraud sucrose yeast extract broth recorded maximum herbicidal activity. The extracts were induced necrotic lesions as fungal conidia after 24 hours on detached leaves. The effect of temperature on herbicidal activity of mycelial and mycelia free filtrate extracts of chloroform and ethanol from modified fries media and sabouraud sucrose yeast extract broth reveals the extracts could retained the herbicidal activity upto 90 oC and induced necrotic lesions on detached leaves.