Optical pH Sensor Based on Calcon Immobilization Membrane

An optical pH sensor was developed by immobilizing Calcon on a porous cellulosic polymer film. The color of the membrane in acidic to basic medium changes from pink to blue, which can be used for determination of pH by spectrophotometry. The sensor response to the pH changes at two wavelengths 510 and 670 nm was investigated. The optical sensor can be used for repetitive and reversible pH measurement in the pH range of 4-9 with a response time of 5 min at 510 nm. The relative standard deviation (R.S.D) was less than 0.51% for seven times alternative measurements of pH from 7 to 8. The sensor was successfully applied to the determination of pH in tap and waste water samples