Genetic Characterization of Sudanese Baggara Cattle Using PCR-RFLP Technique

This study was conducted to identify polymorphisms in promoter and coding regions of the Baggara cattle growth hormone (GH), growth hormone receptor (GHR) and insulin-like growth factor-1 (IGF- 1) genes to distinguish between these cattle sub-types according to differences on these regions. Amplified fragments for GH, GHR and IGF-1 genes were found to be similar in all Baggara cattle subtypes. Polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) genotyping for GH and IGF-1 exon 5 and 1 respectively indicated that Baggara cattle sub-types were similar for these regions of genes. Compared to temperate cattle from other studies the GH at exon 5 of Baggara cattle was found to be different.