Investigation of Ultra Structural Changes in Testicles of German Shepherd Dogs Following Alloxan Induced Diabetes Mellitus

Diabetes mellitus is one of the most common diseases of endocrine glands that is diagnosed by malfunction in natural metabolism of carbohydrate, fat and protein. This disease involves most tissues of the body and the consequent deficiencies reduce their efficiency, cause infections and malfunction in different organs of the body. Alloxan is a chemical substance which is used in creating experimental diabetes in animals. In this investigation, 9 healthy German shepherd dogs were used, five of which were considered as the experimental group and the remaining four were considered as the control group. The necessary examinations were conducted to guarantee their health and they approved to be the absence of diabetes free with intravenous Glucose Tolerance Test (IVGTT). In this study, the experimental group was I intravenously injected with Alloxan monohydrate (lOOmg/kg). Induced diabetes was approved by IVGTT. From the experimental group, showing signs of the death and from the control group (after end of experiment) specimens were taken from testis and processed for microscopic and ultra structural examinations spermatogonia showed vacuolization of cytoplasm and cell degeneration. Spermatogenesis process was faced derangement in testicles and was stopped in one of them. It was concluded that Alloxan Induced Diabetes Mellitus, may cause detrimental effects on Spermatogenesis and testicle function in dogs.