Genetic Diversity Assessment Across Different Genotypes Of Mungbean And Urdbean Using Molecular Markers

Pulses compliment the daily diet of Indians along with cereals. They are rich in proteins with satisfactory proportion of carbohydrates. Mungbean, Vigna radiata and Urd bean, Vigna mungo are the important grain legume crops in agriculture, particularly in India. MYMV (Mungbean Yellow vein Mosaic Virus) is a virus transmitted by whitefly, Bemesia tabaci, the most serious disease of Mungbean and Urdbean. In this study, six each of MYMV resistant and susceptible genotypes in Mungbean and Urbean respectively were selected for the diversity analysis using molecular markers. Twenty four RGA primers from cowpea were used to screen the twenty four genotypes. Dendrogram generated clearly indicated two big clusters at 15% similarity. All mungbean genotypes made one cluster (cluster A) except PS16, which was included in other cluster made by Urdbean genotypes (cluster B). Cluster A contained eleven genotypes while cluster B contained thirteen genotypes. Cluster A and B were further classified into two sub clusters namely A1 and A2, B1 and B2 respectively. A1 consisted of seven genotypes of which five were resistant (PANT MUNG 1, PANT MUNG 5, HUM 12, PUSA 9531, HUM 1) and two were susceptible (TARM 2, KOPERGAON 3), while A2 comprised of remaining four genotypes in which three were susceptible (TAP 7, SML 134 and SML 668), and one (AKM 8803) was resistant. Further, it was found that four mungbean resistant genotypes of A1 namely Pant Mung1, Pant Mung5, HUM 12, and PUSA 9531 made one cluster at 55% similarity. Cluster B, again was subdivided into B1 and B2. B1 consisted a single genotype which was a cross between IPU 99-25* SPS5 while, B2 consisted of the rest of the twelve genotypes. It was interesting to see that two resistant (IPU 02-33 and IPU 6-02) and two susceptible (LBG 20 and T9) genotypes of Urd bean made separate cluster with a similarity of 99 per cent and which indicated that though genotypes are differing at resistant locus, they are highly similar at all other loci.