Polypyrrole Layers Electrodeposited from TRIS Buffer Solution as Matrix of Tyrosinase Biosensor

A novel supporting electrolyte – tris(hydroxymethyl)aminomethane hydrochloride (TRIS) buffer solution was used to obtain polypyrrole (PPy) layers based on the anodic oxidation of pyrrole. Influence of some parameters: temperature, monomer and supporting electrolyte concentration, polarization potential and hydrodynamic conditions on electrodeposition process were investigated. Obtained layers were preliminary tested in construction of an amperometric biosensor based on enzyme tyrosinase, which response was tested using catechol as a substrate.