Imatinib Quantification in Human Serum for Clinical Purposes using High-performance Liquid Chromatography with a Diode Array Detector

The popularity of imatinib in modern medicine has underscored the need for a fast and effective quantification method without the use of expensive instruments. The aim of this study was to develop and validate a relatively fast, cheap and effective HPLC method for the quantification of imatinib in human serum. This method uses a simple preparation step with an ExtrelutR NT 3 extraction tube and commercial available solvents. A Marcherey-Nagel Lichrospher 100-5 RP8 250 × 4 mm column held at 30 °C, a mobile phase of 0.05 M H3PO4/KH2PO4-acetonitrile (7:3, v/v) at a flow rate of 1 mL/min and a diode array detector operated at a wavelength of 265 nm were used for the analysis of 50 μL prepared sample injected into the HPLC. A single run was completed in 15 min. The method presented here has a limit of quantification of 30 ng/mL and is linear between 0.1 and 10 μg/mL.