A Convenient Methods for Synthetic Isomeric Structures of Pyrimido-1,2,4-triazine Derivatives as Biocidal Agents

Some new isomeric structures of pyrimido[2,1-c][1,2,4]triazines 4–8 and 10–14 have been synthesized via the ring closure reactions of 2-hydrazinyl-1-methylpyrimidine 3 and/or 2-hydrazinopyrimidine 9 with acyclic and cyclic oxygen compounds under various conditions. Structures of the targets have been established from their elemental analyses and spectral data (UV, IR, 1H/13C NMR and mass spectrometry). Most of the obtained compounds were evaluated as antimicrobial agents and compared with pipericillin and mycostatine as standard antibiotics. Only compound 7 had highly biocidal effects.