Study of Direct Adventitious Shoot Regeneration in Pomegranate (Punicagranatum cv. MalasSaveh) through Cotyledonary Explants

Punicagranatum cv. Malassaveh is one of the most desirable commercial cultivars in Iran. In this study some experiments were conducted to introduce the best hormonal treatments for direct adventitious shoot regeneration from mentioned cultivar via cotyledonary explants. For this purpose, both liquid and agarMurashige and Skoog (1962) (MS) medium supplemented with BA at 2, 4.5, 9, 13 ?M alone and in combination with 5.5 ?M NAA or 5 ?M 2,4-D were used. The highest number of shoot per explant 8.2 and 7.9 were obtained in MS liquid and agar medium supplemented with 13?M BA and 5.5 ?M NAArespectively, although there was no significant differences between two medium. The highest shoot length 1.65 cm was obtained from MS liquid medium supplemented with13?M BA and 5.5 ?M NAA as well. The highest rooting was obtained in half-strength MS containing 2 mg/l IBA with 3.1 roots per explant. Rooting plantlets were transferred to Jiffy and acclimatized after 35 days successfully.