In Vitro Penetration Rate and Efficacy of Trois in Arthritis Induced Rat Model

The study was performed to evaluate the comparative efficacy of trois with other anti-inflammatory drugs in arthritis induced rat model. Total fifty four wister rats were selected and divided into nine groups of six rats each. Arthritis was induced in knee joint cavity of the right hind leg by intra-articular injections of 0.1mL of 1 % carrageenan in all the groups except control group for 10 days. The control (normal) group was injected with 0.1 ml of saline in knee joint cavity of the right hind leg . After induction of arthrits in all groups, the treatement was started with respective drugs twice a day for 10 days in all groups except control and arthritis induced without treatment group. The blood samples were collected at the end of experiment and various biochemical parameters such as total protein (TP), uric acid, hemoglobin (Hb) and alkaline phosphatase (ALP) were estimated. Antioxidant enzymes such as catalase along with free radical mediate damage malonldialdehyde (MDA) were also measured in the blood sample of all groups. Our finding showed that the level of all biochemical parameters were significantly increased as well as MDA level along with decreased level of hemoglobin and catalase enzyme activity in arthritis induced group. After treatment with respective drugs, all biochemical parameters along with free radical mediated damage MDA levels were significantly improved in all treated groups as compared with arthritis induced group. When trois treated group was compared with all other treated groups, these biochemical parameters were showed better improvement in trois treated group. On the basis of findings, we concluded that trois is effective anti-inflammtory drug that improved inflammation, pain along with biochemical alteration and MDA level during arthritis