Identification of RAPD Primers Specific for Wheat Homoeologous Group 1 and 3 Chromosomes

Mapping of homoeologous chromosomes of common wheat (Triticum aestivum L.) has always been a top priority for wheat breeders. During present work, easier, cheaper and quicker RAPD (Randomly Amplified Polymorphic DNA) primers were mapped on homoeologous group 1 and 3 chromosomes of common wheat. Wheat genetic stocks viz; Nullisomic-tetrasomic lines were used for mapping of RAPD primers. A total of 43 RAPD primers were tested, out of which, five primers viz; GLA-10, GLA-11, GLA-14, GLA-20 and GLB-15 were found useful and these primers amplified DNA fragments specific for chromosomes 1D, 3D, 1B, 3B and 1D, respectively. These five RAPD primers can reliably be used as markers for the wheat homoeologous chromosomes 1B, 3B, 1D and 3D.