HIGHLIGHT OF BUFFALO MILK CHOLESTEROL THROUGH THIN-LAYER CHROMATOGRAPHY

The highlight of buffalo milk cholesterol was conducted by means of thin-layer chromatography and the migration of mobile phases along an absorbent layer. Free and esterised cholesterol extraction was conducted according to the method of Folch et. al., performing small alterations regarding solvent volume or the volume of samples under analysis. Many reactives underwent testing, as the initial iodine visualising was not appropriate. The quantitative identification was conducted by comparing the Rfs of free and esterised cholesterol from analysed samples with existing standards. Spots were highlighted using a photodensimeter