Prevalence and Antibiogram Studies of Salmonella Enteritidis Isolated from Human and Poultry Sources

A total of 615 samples including poultry eggs (240), poultry meat (85), poultry droppings (100), bakery products (65) and stool from human diarrhea cases (125) were collected from different areas of Faisalabad, Pakistan. The samples enriched in Tetrathionate broth showed turbidity in 206 (33.5%) samples, and typical Salmonella colonies were found on differential agar media by using traditional methods in all these cases. Polyvalent antisera were used to identify Salmonella serovars. Results revealed that overall serovar entertidis prevalence rate in 206 Salmonella positive samples was 75.24% (155). Out of 58 isolates of Salmonella recovered from human stool samples, 44 (75.86%) were S. enteritidis. Isolation frequency of S. enteritidis from total isolates (148/206) in poultry sources was 111/148 (75.00%) which indicated the zoonotic potential of S. enteritidis. Antimicrobial susceptibility tests were performed by using disk diffusion and concentration methods. S. enteritidis isolates showed 100% resistance against bacitracin, erythromycin and novobiocin. These results indicated possible role of infected poultry and poultry products as a source of human infection with multiple drug resistant S. enteritidis strains