Title of article :
Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization
Author/Authors :
D.Y. Zhang، نويسنده , , J.Y. Zhou، نويسنده , , W.Q. Chen and B.S. Wang، نويسنده , , J.G. Chen and H. Zhang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Abstract :
The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Vero cells, and the expression of targeted proteins was confirmed by an indirect immunofluorescent assay and immunocyto-chemistry assay. By repeating intramuscular injection several times, the co-expression plasmids provided chicks with some immune protection against viral infection
Keywords :
IBV , S1 gene , expression , IL2
Journal title :
Veterinarni medicina
Journal title :
Veterinarni medicina
