Author/Authors :
Ingec، Metin نويسنده Obstetrics and Gynecology Department, Faculty of Medicine, Atatürk University, Erzurum, Turkey , , Calik ، Muhammet نويسنده Department of Pathology, Medical Faculty, Ataturk University, Erzurum, Turkey , , Gundogdu، Cemal نويسنده Department of Pathology, Medical Faculty, Ataturk University, Erzurum, Turkey , , Kurt، Ali نويسنده Department of Pathology, Education and Research Hospital, Erzurum, Turkey , , Yilmaz، Mehmet نويسنده Nene Hatun Obstetrics and Gynecology Hospital, Erzurum, Turkey , , Isaoglu، Unal نويسنده Nene Hatun Obstetrics and Gynecology Hospital, Erzurum, Turkey , , Salman، Suleyman نويسنده Obstetrics and Gynecology Hospital, Ministry of Health, Igd?r, Turkey , , Akcay ، Fatih نويسنده Department of Biochemistry, Medical Faculty, Ataturk University, Erzurum, Turkey , , Suleyman، Halis نويسنده Pharmacology Department, Faculty of Medicine, Atatürk University, Erzurum, Turkey ,
Abstract :
Background: The effects of moclobemide on damaged ovarian tissue induced by ischemia-reperfusion
and damaged contralateral ovarian tissue were investigated in rats,
biochemically
and histologically.
Materials and Methods: In this experimental study, 40 rats were equally divided into four groups: 10 mg/kg moclobemide, 20 mg/kg moclobemide, ischemia/reperfusion control,
and intact control
groups. A
2-2.5-cm-long vertical incision
was made in the lower abdomen
of
each rat
in
order
to
reach
the
ovaries,
after
which
a
vascular
clip
was
placed on
the
lower
side
of
the
right
ovary
of
each
animal
in
the
two
treatment
groups
and the
ischemia-reperfusion
control
group,
but not
in
the
healthy
(intact
control)
animal
group.
The
purpose of this procedure was to
create ischemia over the course of three hours, then
the
clips were
unclamped to
provide reperfusion for the next two hours. At
the end
of the
two hours of reperfusion, all the animals were
killed by
high-dose anaesthesia and
their
ovaries
were
taken and
subjected to
histological
and
biochemical
(malondialdehyde,
nitric
oxide, glutathione) studies.
Results: The obtained results showed that moclobemide suppressed nitric oxide and malondialdehyde production in the ischemia - reperfusion damage area, and prevented
the decrease in endogenous antioxidant levels (glutathione) in the rat ovarian
tissue.
Moclobemide also prevented infiltration of leukocytes to the ovarian tissue. These results showed that moclobemide protected ovarian tissue against ischemiareperfusion
injury.
Conclusion: This study shows that moclobemide represses malondialdehyde and nitric oxide production in the rat ovarian tissue subjected to ischemia-reperfusion injury and keeps the endogenous antioxidant glutathione level from decreasing. Moclobemide also inhibits leukocytic migration into ovarian tissue following ischemia-reperfusion injury. From these results, it is suggested that moclobemide can be used in the treatment of ovarian
ischemia-reperfusion injury.
