Partial Characterization of Hemolymph of Different Spider Species of Citrus Orchards

Erythrocyanin a toxic protein present in the hemolymph. The hemolymph is composed of low and high molecular weight components, while toxins constituting a very small portion. The spider species including in this study were Myrmarachne maratha, M. orientales, M. bengalensis, M. laetus, M. japonica, Phidippus workmani, Thyene imperialis, Plexippus paykulli, Phintella sp., P. castriesiana, Pelegrina verecunda, Cheiracanthium mildei, Elaver sp., Stegodyphus sarasinorum Heteropoda kandiana, Olios lutescens, O. flavidus and O. giganteus. Free ions, biochemical properties were studies. P. verecunda and C. mildei showed lower ion concentration. Family Sparassidae (pooled) had Na+ concentration and S. sarasinorium showed high Ca2+ concentration. Genus Myrmarachne (pooled) had lower protein concentration but high casein hydrolization. Elaver sp. had high protein contents but moderate casein and gelatin activity. SDS-PAGE revealed 200 kDa and 60 kDa bands were present in all species. This study can be helpful in further advancement in drug development and other pharmaceuticals. © 2010 Friends Science Publishers