Author/Authors :
Karimianpour، Navaz نويسنده , , Mousavi-Shafaei، Parisa نويسنده , , Ziaee، Abed-Ali نويسنده , , Akbari، Mohammad نويسنده , , Pourmand، Gholamreza نويسنده , , Abedi، Amirreza نويسنده , , Ahmadi، Ali نويسنده Department of Pathology, Sina Hospital, Tehran University of Medical Sciences, Tehran , , Alavi، Hossein Afshin نويسنده Department of Pathology, Day Hospital, Tehran ,
Abstract :
Introduction: Studies have shown different types of RAS mutations in human bladder tumors with a wide range of mutation frequencies in different patient populations. This study aimed to assess the frequency of specific-point mutations in the RAS gene family of a group of Iranian patients with bladder cancer. Methods: We examined the tumor specimens of 35 consecutive patients with transitional cell carcinoma. The DNA samples were evaluated for the occurrence of HRAS, KRAS, and NRAS activation using a polymerase chain reaction-restriction fragment length polymorphism technique. Results: None of the patients had mutations in the RAS gene family “hot spots” including codons 12, 13, and 61. Conclusion: We failed to find RAS mutations in our bladder tumor samples. These observations may reflect the involvement of different etiological factors in the induction of bladder tumor of which RAS mutation might not be present in all populations.
