Author/Authors :
Sadat Hosseini، Motahare نويسنده Biomedical Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, IR Iran Sadat Hosseini, Motahare , Tazzoli-Shadpour، Mohammad نويسنده Biomedical Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, IR Iran Tazzoli-Shadpour, Mohammad , Amjadi، Issa نويسنده Biomedical Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, IR Iran Amjadi, Issa , Haghighipour، Nooshin نويسنده National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran , , Shokrgozar، Mohammad Ali نويسنده , , Ghafourian Boroujerdnia، Mehri نويسنده Immunology Department, Medical College, Ahvaz Jundishapur University of Medical Science, Ahvaz, IR Iran Ghafourian Boroujerdnia, Mehri
Abstract :
Substrates in medical science are hydrophilic polymers undergoing volume
expansion when exposed to culture medium that influenced on cell attachment.
Although crosslinking by chemical agents could reduce water uptake and promote mechanical
properties, these networks would release crosslinking agents. In order to overcome
this weakness, silicone rubber is used and reinforced by nanoclay.
Objectives: Attempts have been made to prepare nanocomposites based on medical
grade HTV silicone rubber (SR) and organo-modified montmorillonite (OMMT) nanoclay
with varying amounts of clay compositions.
Materials and Methods: Incorporation of nanocilica platelets into SR matrix was carried
out via melt mixing process taking advantage of a Brabender internal mixer. The
tensile elastic modulus of nanocomposites was measured by performing tensile tests on
the samples. Produced polydimetylsiloxane (PDMS) composites with different flexibilities
and crosslink densities were employed as substrates to investigate biocompatibility,
cell compaction, and differential behaviors.
Results: The results presented here revealed successful nanocomposite formation with
SR and OMMT, resulting in strong PDMS-based materials. The results showed that viability,
proliferation, and spreading of cells are governed by elastic modulus and stiffness
of samples. Furthermore, adipose derived stem cells (ADSCs) cultured on PDMS and
corresponding nanocomposites could retain differentiation potential of osteocytes in
response to soluble factors, indicating that inclusion of OMMT would not prevent osteogenic
differentiation. Moreover, better spread out and proliferation of cells was observed
in nanocomposite samples.
Conclusions: Considering cell behavior and mechanical properties of nanobiocomposites
it could be concluded that silicone rubber substrate filled by nanoclay are a good
choice for further experiments in tissue engineering and medical regeneration due to
its cell compatibility and differentiation capacity
Implication for health policy/practice/research/medical education:
Improved mechanical properties of nanobiocomposites result in proper cell response through adjustment and arrangement of
cytoskeletal fibers. Results can be applied in tissue engineering when enhanced substrates are required for in vivo improvement
of cell behavior.
