Author/Authors :
Behfar، Abdolazim نويسنده Department of Food Science and Medical Hydrology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Behfar, Abdolazim , Nazari Khorasgani، Zahra نويسنده Department of Pharmacology and Toxicology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Nazari Khorasgani, Zahra , Alemzadeh، Ziyaaddin نويسنده Department of Food Science and Medical Hydrology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Alemzadeh, Ziyaaddin , Goudarzi، Mehdi نويسنده Department of Pharmacology and Toxicology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Goudarzi, Mehdi , Ebrahimi، Rezvan نويسنده Department of Pharmacology and Toxicology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Ebrahimi, Rezvan , Tarhani، Najmedin نويسنده Department of Food Science and Medical Hydrology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Tarhani, Najmedin
Abstract :
Aflatoxins are one of the most potent toxic substances that occur naturally.
Nowadays extensive attention has been taken to their existence in food and environment,
as there is the possibility of harm to humans following chronic exposure to
extremely low levels via food chain. Aflatoxin M1 (AFM1) is a hepatic carcinogenic metabolite
found in the milk of lactating animals fed with contaminated feed contaminated by
aflatoxin B1 (AFB1).
Objectives: This study aimed to determine the levels of AFM1 in produced pasteurized
milk in the Ahvaz of city.
Materials and Methods: For this purpose, 100 samples of pasteurized milk from the Jamus
Factory were analyzed the to determine AFM1 content by using an immunoaffinity
column for clean-up and high-performance liquid chromatography (HPLC) with a C18
column, a fluorescence detector (excitation 365 nm, emission 435 nm) and a mobile
phase of acetonitrile–water (25:75, v/v) at a flow rate of 1 mL/min.
Results: AFM1 was detected in all 100 samples of pasteurized milk at concentrations
ranging from 0.45 to 9.760 ng/L.
Conclusions: The mean concentration of AFM1 in the the pasteurized milk samples was
2.7 ng/L, which was below the 50 ng/L, accepted as level of for milk in Iran.
