Author/Authors :
Wu، Min نويسنده Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou 225003, CHINA , , Qing-lianc، Ge نويسنده Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou 225003, CHINA , , Gao، Yu-shi نويسنده Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou 225003, CHINA , , Chen، Kuan-wei نويسنده Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou 225003, CHINA ,
Abstract :
A method to quick detect concentration of lecithin in eggs, namely microwave digestion spectrophotometry, was established in this research. The homogenate of eggs was treated with absolute ethanol to eliminate phosphate protein in eggs which could possibly affect concentration of lecithin examined. A sample then received a new way of pre-treatment, called microwave digestion, before UV-Vis spectrometry was applied to examine the concentration of phosphate at 400nm. The linear equation was A = 0.08628X (?g), the corresponding coefficient of correlation was 0.9998,the detection limit of phosphorous was 0.2?g (n=11). The content of lecithin in eggs was then obtained. According to the result, the recovery of 90% was secured; therefore
the conclusion of high degree of accuracy was reached.
