Author/Authors :
Alipour، Razieh نويسنده Bs, Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan , , Sadeghi، Farzaneh نويسنده , , Hashemi-Beni، Batool نويسنده PhD, Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan , , Zarkesh-Esfahani، Sayyed Hamid نويسنده PhD, Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan , , Heydari، Fariba نويسنده DDS, Torabinegad Research Center, Dental School, Isfahan University of Medical Sciences, Isfahan , , Mousavi، Sayyed Behrouz نويسنده DDS, Torabinegad Research Center, Dental School, Isfahan University of Medical Sciences, Isfahan , , Adib، Minoo نويسنده , , Narimani، Manizheh نويسنده MSc, Central lab, Medical School, Isfahan University of Medical Sciences, Isfahan , , Esmaeili، Nafiseh نويسنده MSc, Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan ,
Abstract :
Objectives: Mesenchymal stem cells or ‘‘multipotent stromal
cells’’ are heterogeneous cell population with self-renewal and
multi-linage differentiation. The aim of this study was to examine
and compare the expression of important stem cell surface markers
on two populations of mesenchymal stem cells, one derived
from human exfoliated deciduous teeth and the other derived
from human adipose tissue. These new stem cells will offer a
promising avenue for prevention and reversal of many human
diseases such as type 1 diabetes and prevention of liver fibrotic
process.
Methods: Mesenchymal stem cells were isolated and cultured
from human adipose tissue and dental pulp of human exfoliated
deciduous teeth. The cultured cells then were harvested and
stained by different fluorescent labeled monoclonal antibodies
against surface markers and were analyzed using flow cytometry.
Results: Both different cell populations expressed CD44, CD90
and CD13 (stem cell markers) with similar intensity. They did not
express hematopoietic markers (CD11b, CD19 and CD34), and
lymphocyte or leukocyte antigens CD3, CD7, CD20, CD14,
CD45, CCR5 (CD195), CD11b and CD10 on their surfaces. Two
different cell types demonstrated different levels of expression in
CD56 and CD146. Mesenchymal stem cells from human exfoliated
deciduous teeth were positive for CD105 and were negative
for CCR3 and CCR4 expression.
Conclusions: Both cell populations derived from adipose tissue
and dental pulp showed common phenotypic markers of mesenchymal
stem cells. In conclusion, mesenchymal stem cells could be
isolated and cultured successfully from dental pulp of human exfoliated
deciduous teeth, they are very good candidates for treatment
and prevention of human diseases.
