Author/Authors :
Shareghi، B نويسنده Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran , , Farhadian، S نويسنده Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran , , Salavati-Niasari، M نويسنده Institute of Nano Science and Nano Technology, University of Kashan,Kashan,P.O.BOX.87317-51167,I. R. of Iran ,
Abstract :
The interaction between nano-SiO2
and lysozyme was investigated by
the method of UV-Visible detection and fluorescence spectroscopic
techniques. The thermal denaturation of lysozyme has been
investigated in the presence and absence of nano-SiO2
over the
temperature range (293-373) K in different buffers and pH values,
using temperature scanning spectroscopy. The presence of nano-SiO2
caused the destabilization of lysozyme resulting in a decrease in the
temperature of unfolding with an increase in nano-SiO2
concentration.
