Author/Authors :
Shareghi، B نويسنده Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran , , Nooraei، P نويسنده Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran , , Salavati-Niasari، M نويسنده Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O.BOX.87317-51167,I. R. of Iran , , Shahbazkia، H. R نويسنده Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran , , Semnani، A نويسنده Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran ,
Abstract :
The interaction of copper oxide nanoparticles and copper ions and
proteinase K from Tritirachium album Limber has been investigated
employing UV spectroscopy and kinetics measurements. The aim of
this study was to evaluate the effect of nanoparticles of CuO for
proteinase K. In this paper we compare the effect of copper oxide
nanoparticles with the effect of copper ions on proteinase K stability
and activity. Copper oxide nanoparticles had more inhibitory and
destabilization effect than copper ions on proteinase K. We used
thermodynamic parameter such as Tm and ? G
0
and kinetic studies to
emphases the effect of nanoparticles CuO and copper ions on
stability and activity of proteinase-K.
