Genotypic and Allelic Frequencies of Genes in Broilers Analysed by Using Techniques

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broiler chickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and di-gested with HinfI and Hsp92II restriction enzymes, respectively. Two types of alleles of A and B and three types of genotypes of AA, AB and BB were observed in both genes. The results indicated predominant B-allele (0.85) was higher for IGF1 but A-allele (0.5611) IGF2in the broiler population.