Author/Authors :
Shadkhast، Mohammad نويسنده , , Shabazkia، Homayoun-Reza نويسنده Department of Biochemistry and Pharmacology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran , , BIGHAM SADEGH، Amin نويسنده , , Shariati، Seyed Ebrahim نويسنده Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran , , Mahmoudi، Taji نويسنده Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran , , Shariffian-Fard، Mojdeh نويسنده PhD Student of Bacteriology, Faculty of Veterinary Medicine, University of Ghent, Ghent, Belgium ,
Abstract :
In this study morphological characterization of leukocyte and erythrocyte were investigated in central Asian tortoise (Testudo horsfieldii) in Iran. 7 males and 7 females were used in this study. Erythrocyte and leukocyte morphology were described using Wright´s technique. The sizes of erythrocyte and their nuclei were measured using an ocular micrometer under objective lenses (100x). Results of this study were compared with previous work on the other reptile species. Because there were no significant differences between the erythrocyte size of female and male tortoise, the data from the females and males of individual species were pooled. The mean length of mature erythrocytes was 1.98 ?m (± 0.16 SD, with a range 1.10 - 2.50 ?m).The mean width of mature erythrocytes was 1.10 ?m (± 0.14 SD, with a range of 0.80 - 1.90 ?m).The mean length of nucleus was 0.71 ?m (± 0.09 SD, with a range of 0.50 - 1.00 ?m). The mean width of the nucleus was 0.52 ?m (± 0.07 SD, with a range of 0.40 - 0.70?m). Five types of leukocytes were found in the tortoise blood namely basophiles, eosinophils, lymphocytes, heterophils and monocytes.
