Effect of methyl jasmonate and salicylic acid on noradrenalin accumulation in hairy roots of Portulaca oleracea L.

ABSTRACT: Portulaca oleracea L. is a medicinal plant find in Europe and Asia. This plant contains valuable secondary metabolite such as noradrenalin. Naturally the wild plant produce small amount of this secondary metabolites so induction of hairy roots can be useful to increase its production. In our research Agrobacterium rhizogenes strain AR15834 was used to induce hairy roots in the two-week leaf explants of P. oleracea. PCR analysis with specific primers rolB gene was performed to confirm the transgenic hairy roots production. PCR analysis results showed diagnostic bands with size 780 bp related to specific reproduction of rolB gene, so the transgenic hairy roots was confirmed. The effect of two chemical elicitors, salicylic acid and methyl jasmonate, on the production of noradrenalin in P. oleracea hairy roots was examined. Methyl jasmonate was found to increase the production of noradrenalin. The optimal methyl jasmonate dose was between 100um and 200um for hairy roots harvested 48h after elicitation. After 48h of induction with 200um methyl jasmonate, an eightfold increase in the level of noradrenalin was observed in compared with control cultures.