Author/Authors :
Sezginturk، Mustafa Kemal نويسنده , , Alaçam، Sencer نويسنده Ege University, Faculty of Science, Biochemistry Department35100,Bornova- ?zmir/Turkey , , Altu?، Ca?r? نويسنده Ege University, Faculty of Science, Biochemistry Department35100,Bornova- ?zmir/Turkey , , Akbayirli، P?nar نويسنده Ege University, Faculty of Science, Biochemistry Department35100,Bornova- ?zmir/Turkey , , Dinckaya، Erhan نويسنده ,
Abstract :
In this study, we developed a hybrid biosensor based on sulfite oxidase and
glucose oxidase for determination of sulfite and glucose. The principle of the measurements
was based on the determination of the decrease in the differentiation of oxygen level which
had been caused by the catalytic reactions of the enzymes in the bioactive layer of the
biosensor. The biosensor showed a linear response for an interval of sulfite concentration
between 1×10-4 and 1.75×10-3 M and for glucose concentrations between 2.5×10-5 and
1.25×10-3 M. After some optimum and characterization studies, the proposed biosensor was
applied to the determination of sulfite and glucose in certain real samples, blood and pickle
water.
