Author/Authors :
A، Das نويسنده Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam-638401, Erode District, Tamil Nadu, India , , Y، Mazumder نويسنده Epsilon Institute of Clinical Science, Mangalore-575003, Karnataka, India , , B. K، Dutta نويسنده Department of Ecology and Environmental Science, Assam University, Silchar-788011, Assam, India , , B. R، Shome نويسنده Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal-560024, Karnataka, India , , K. M، Bujarbaruah نويسنده Assam Agriculture University, Jorhat-785013, Assam, India ,
Abstract :
Diarrhoea is the outward sign of a gut abnormality both in animal and human and should always be treated as potentially serious. Present research investigation was carried out to identify the prevalent genotypes of Clostridium perfringens associated with the diarrhea in cattle. A total of 22 isolates of C. perfringens were biochemically identified from the faecal samples collected from 34 cattle suffering from diarrhoea maintained in a semi intensive rearing system of organized dairy farms in Meghalaya, India. Polymerase chain reaction (PCR) was employed with alpha toxin gene (cpa), beta toxin gene (cpb), epsilon toxin gene (etx), iota toxin gene (iA), enterotoxin gene (cpe) and beta2 toxin genes (cpb2) primers to identify the specific toxin types of C. perfringens. All the 22 isolates were positive for only cpa gene. None of the isolates were found positive for any other toxin genes. The PCR results revealed that all the isolates from the diarrhoeic cattle belonged to the genotype A. This result suggested that type A is the most frequently isolated genotype of C. perfringens present in the intestines of cattle. PCR has proven a reliable and sensitive diagnostic tool for the rapid identification of C. perfringens.
