Author/Authors :
Irvani، N. نويسنده Department of biotechnology, Faculty of new technologies and energy engineering, Shahid Beheshti University, G.C., Evin, Tehran, Iran , , Solouki، M. نويسنده Department of Agronomy and Plant Breeding, Faculty of Agriculture, Zabol University, Sistan, Iran. , , Omidi، M. نويسنده , , Al-Saidi، I. A. نويسنده , , Zare، A. R. نويسنده Department of biotechnology of medicinal plans, Institute of Medicinal Plants, ACECR, Karaj, Iran. ,
Abstract :
Dorema ammoniacum D. Don. (Apiaceae) which is native to Iran is classified as a vulnerable
medicinal species. The aim of this study was to enhance the germination of Dorema ammoniacum
seeds, which have a low percentage under normal laboratory conditions. Different pretreatments
were carried out to obtain efficacy in breaking dormancy and speeding germination, using of
scarification, cold stratification, Washing and chilling treatments, gibberellic acid (GA3) and N6-
benzyladenine (BA) treatments. The rate and percentage of seed germination varied with duration
and medium of stratification. The highest germination percentage was obtained in the moist sand
cold stratification for 45 days (68.00 ± 1.7). Both germination rate and percentage of Dorema
ammoniacum increased by increasing the number of exposure days at 4 °C. However, when the
chilling temperature was decreased from 4 to -5 and -10 °C, a negative response was monitored for
both germination rate and percentage. Addition of GA did not promote germination. Regardless of
applied treatment to seeds, germination rate was positively correlated with germination percentage.
Therefore, fast germination was associated with high germination percentage. For the first time,
these results indicated that Dorema ammoniacum seeds which have deep morphophysiological
dormancy need a chilling treatment for reliable germination.
